Parallel genetics of regulatory sequences using scalable genome editing in vivo.

Froehlich JJ, Uyar B, Herzog M, Theil K, Glazar P, Akalin A, Rajewsky N
Cell Rep. 2021 Apr 13;35(2):108988. doi: 10.1016/j.celrep.2021.108988. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
163862	pJJF152_Phsp_Cas9	heat shock-induced expression of Cas9 in C. elegans
163864	pJJF143_SECGFP_sg1	sgRNA targeting GFP in C. elegans. Backbone: pJJR50.
163865	pJJF144_SECGFP_sg2	sgRNA targeting GFP in C. elegans. Backbone: pJJR50.
163866	pJJF449_dpy-10_CDS_sg1	sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.
163867	pJJF328_sqt-3_3′UTR_sg2	sgRNA targeting sqt-3 (3′UTR) in C. elegans. Backbone: pJJR50.
164266	pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone	sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.
164267	pJJF496_dpy-10_CDS_sg6	sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJR50.
164268	pJJF495_dpy-10_CDS_sg6	sgRNA targeting dpy-10 (CDS) in C. elegans. Backbone: pJJF439.