A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre C, de Los Santos EL, Freemont PS
ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163756 | pTU1-A-SP44-mScarlet-I | Expresses Streptomyces codon-optimised mScarlet-I |
| 163757 | pTU1-A-SP44-sfGFP | Expresses Streptomyces codon-optimised sfGFP |
| 163763 | pTU1-A-SP44-mVenus-I | Expresses Streptomyces codon-optimised mVenus-I |
| 172430 | pTU1-B-SP44s-GUS | Expresses Streptomyces codon-optimised beta-D-glucuronidase |
| 172431 | pSF1C-A-RFP | StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069) |
| 172432 | pSF2C-A-RFP | StrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069) |