A suppressor tRNA-mediated feedforward loop eliminates leaky gene expression in bacteria.
Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR
Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163718 | pJH474_pTech_Para-supP | Arabinose-inducible expression of supP |
| 163719 | MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG | Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine |
| 163720 | pJH25_pBad_AraC_Para-sfGFP.0TAG | Arabinose-inducible expression of green fluorescent protein encoding zero amber codons |
| 163721 | pJH26_pBad_AraC_Para-sfGFP.1TAG | Arabinose-inducible expression of green fluorescent protein encoding one amber codon |
| 163722 | pJH27_pBad_AraC_Para-sfGFP.2TAG | Arabinose-inducible expression of green fluorescent protein encoding two amber codons |
| 163723 | pJH28_pBad_AraC_Para-sfGFP.3TAG | Arabinose-inducible expression of green fluorescent protein encoding three amber codons |
| 163724 | pJH625_pJH_LldR_PlldR-sfGFP.0TAG | Lactate-inducible expression of green fluorescent protein encoding zero amber codons |
| 163725 | pJH626_pJH_LldR_PlldR-sfGFP.1TAG | Lactate-inducible expression of green fluorescent protein encoding one amber codon |
| 163726 | pJH627_pJH_LldR_PlldR-sfGFP.2TAG | Lactate-inducible expression of green fluorescent protein encoding two amber codons |
| 163727 | pJH628_pJH_LldR_PlldR-sfGFP.3TAG | Lactate-inducible expression of green fluorescent protein encoding three amber codons |
| 163728 | pJH674_pTech_PlldR-HHRz-supP | Lactate-inducible expression of supP, preceded by a hammerhead ribozyme |
| 163729 | pJH721_pJH_LldR_PlldR-sfGFP.20TAG | Lactate-inducible expression of green fluorescent protein encoding twenty amber codons |
| 163730 | pJH726_pJH_LldR_PlldR-sfGFP.15TAG | Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons |
| 163731 | pJH727_pJH_LldR_PlldR-sfGFP.10TAG | Lactate-inducible expression of green fluorescent protein encoding ten amber codons |
| 163732 | pJH773_pTech_PlldR-HHRz-supP(opal) | Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme |
| 163733 | pJH774_pJH_LldR_PlldR-sfGFP.1opal | Lactate-inducible expression of green fluorescent protein encoding one opal codon |
| 163734 | pJH775_pJH_LldR_PlldR-sfGFP.2opal | Lactate-inducible expression of green fluorescent protein encoding two opal codons |
| 163735 | pJH776_pJH_LldR_PlldR-sfGFP.3opal | Lactate-inducible expression of green fluorescent protein encoding three opal codons |
| 163736 | pJH805_pJH_LldR_Pcon-sfGFP.0TAG | Constitutive expression of green fluorescent protein encoding zero stop codons |
| 163737 | pJH806_pJH_LldR_Pcon-sfGFP.1TAG | Constitutive expression of green fluorescent protein encoding one stop codon |
| 163738 | pJH807_pJH_LldR_Pcon-sfGFP.2TAG | Constitutive expression of green fluorescent protein encoding two stop codons |
| 163739 | pJH809_pJH_LldR_Pcon-sfGFP.10TAG | Constitutive expression of green fluorescent protein encoding ten stop codons |