Addgene: A suppressor tRNA-mediated feedforward loop eliminates leaky gene expression in bacteria.

A suppressor tRNA-mediated feedforward loop eliminates leaky gene expression in bacteria.

Ho JML, Miller CA, Parks SE, Mattia JR, Bennett MR
Nucleic Acids Res. 2021 Mar 18;49(5):e25. doi: 10.1093/nar/gkaa1179. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
163739	pJH809_pJH_LldR_Pcon-sfGFP.10TAG	Constitutive expression of green fluorescent protein encoding ten stop codons
163738	pJH807_pJH_LldR_Pcon-sfGFP.2TAG	Constitutive expression of green fluorescent protein encoding two stop codons
163737	pJH806_pJH_LldR_Pcon-sfGFP.1TAG	Constitutive expression of green fluorescent protein encoding one stop codon
163736	pJH805_pJH_LldR_Pcon-sfGFP.0TAG	Constitutive expression of green fluorescent protein encoding zero stop codons
163735	pJH776_pJH_LldR_PlldR-sfGFP.3opal	Lactate-inducible expression of green fluorescent protein encoding three opal codons
163734	pJH775_pJH_LldR_PlldR-sfGFP.2opal	Lactate-inducible expression of green fluorescent protein encoding two opal codons
163733	pJH774_pJH_LldR_PlldR-sfGFP.1opal	Lactate-inducible expression of green fluorescent protein encoding one opal codon
163732	pJH773_pTech_PlldR-HHRz-supP(opal)	Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme
163731	pJH727_pJH_LldR_PlldR-sfGFP.10TAG	Lactate-inducible expression of green fluorescent protein encoding ten amber codons
163730	pJH726_pJH_LldR_PlldR-sfGFP.15TAG	Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons

Antibodies from Article