Genome-wide gene expression tuning reveals diverse vulnerabilities of M. tuberculosis.
Bosch B, DeJesus MA, Poulton NC, Zhang W, Engelhart CA, Zaveri A, Lavalette S, Ruecker N, Trujillo C, Wallach JB, Li S, Ehrt S, Chait BT, Schnappinger D, Rock JM
Cell. 2021 Jul 16. pii: S0092-8674(21)00824-2. doi: 10.1016/j.cell.2021.06.033.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163631 | plRL2 | Optimized M. tuberculosis CRISPRi plasmid; L5 integrating. |
| 163632 | plRL118 | Renilla luciferase reporter plasmid; Giles integrating. |
| 163633 | plRL61 | Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634). |
| 163634 | plRL19 | L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633). |
| 163635 | plRL117 | Optimized M. smegmatis CRISPRi plasmid; L5 integrating. |
| 163954 | RLC12 | Targets open reading frames and non-coding RNAs in M. tuberculosis. |
| 163955 | RLC11 | Targets open reading frames and non-coding RNAs in M. smegmatis. |
| 166886 | plRL58 | Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634) |