NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins.

Scheu AHA, Lim SYT, Metzner FJ, Mohammed S, Howarth M
Nat Commun. 2021 Jan 29;12(1):717. doi: 10.1038/s41467-021-20963-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
163613	pET28a-His6-OAZ-GSY-SPM-Ctag	Expresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.
163614	pET28a-His6-ODC-Ctag	Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.
163615	pET28a-TGFα-GSY-SPM	Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.