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Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease.

Qi T, Wu F, Xie Y, Gao S, Li M, Pu J, Li D, Lan F, Wang Y
Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
135974epi-ABEmaxExpresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
135975epi-BE4maxExpresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
135976epi-ABEmax-NGExpresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
135977epi-BE4max-NGExpresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Antibodies from Article