Sanford Simon Lab: Simon lab plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163417 | Nup133_mEGFP(-8a) | Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids) |
| 163418 | Nup133_mEGFP(-8b) | Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids) |
| 163419 | Nup133_mEGFP(-9a) | Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids) |
| 163420 | Nup133_mEGFP(-9b) | Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids) |
| 163424 | Nup58_mEGFP(-7) | Mammalian expression of Nup58 with a mEGFP (missing the first 7 amino acids) at amino acid position 412 |
| 163426 | Nup54-mEGFP494(0) | Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of the mEGFP |
| 163427 | Nup54-mEGFP494(1) | Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of the mEGFP |
| 163428 | Nup54-mEGFP494(2) | Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFP |
| 163429 | Nup54-mEGFP494(flex0) | Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 5 amino acids at the carboxyl end of the mEGFP |
| 163430 | Nup54-mEGFP494(flex1) | Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 6 amino acids at the carboxyl end of the mEGFP |
| 163431 | Nup54-mEGFP494(flex2) | Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 7 amino acids at the carboxyl end of the mEGFP |
| 163432 | Nup54_mEGFP494(-4) | Mammalian expression of Nup54 with a mEGFP (missing the first 4 amino acids) at amino acid position 494 |
| 163433 | Nup54_mEGFP494(-5) | Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 |
| 163434 | Nup54_mEGFP494(-6) | Mammalian expression of Nup54 with a mEGFP (missing the first 6 amino acids) at amino acid position 494 |
| 163438 | BFP-RanQ69L | Mammalian expression of RanQ69L with tag-BFP; contains miniAux domain for degredation |
| 163443 | NLS-tdTomato | Bacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backbone |