An optimized retroviral toolbox for overexpression and genetic perturbation of primary lymphocytes.
van der Donk LEH, van der Spek J, van Duivenvoorde T, Ten Brink MS, Geijtenbeek TBH, Kuijl CP, van Heijst JWJ, Ates LS
Biol Open. 2022 Feb 15;11(2):bio059032. doi: 10.1242/bio.059032. Epub 2022 Mar 1.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163324 | pMX-hPGK-CD90.2-Cd247A_miR | Retroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the human PGK promoter. |
| 163325 | pMX-hFTH1-CD90.2-Cd247A_miR | Retroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the human FTH1 promoter. |
| 163326 | pMX-CAG-CD90.2-Cd247A_miR | Retroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the CAG promoter. |
| 163327 | pMX-mPGK-CD90.2-Cd247A_miR | Retroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the murine PGK promoter. |
| 163328 | pMY-CD90.2-Cd247A_miR | Retroviral vector for knockdown of murine CD247A (TCR zeta) and expression of a CD90.2 surface marker under control of the LTR promoter. |
| 163329 | pMX-hPGK-CD90.2-Rluc_miR | Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the human PGK promoter. |
| 163330 | pMX-hFTH1-CD90.2-Rluc_miR | Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the human FTH1 promoter. |
| 163331 | pMX-CAG-CD90.2-Rluc_miR | Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the CAG promoter. |
| 163332 | pMX-mPGK-CD90.2-Rluc_miR | Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the murine PGK promoter. |
| 163333 | pMY-CD90.2-Rluc_miR | Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the LTR promoter. |
| 163334 | pMX-hPGK-CD3-P2A-CD90.2 | Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the hPGK promoter |
| 163335 | pMX-hFTH1-CD3-P2A-CD90.2 | Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the hFTH1 promoter |
| 163336 | pMX-CAG-CD3-P2A-CD90.2 | Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the CAG promoter |
| 163337 | pMX-mPGK-CD3-P2A-CD90.2 | Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the mPGK promoter |
| 163338 | pMY-CD3-P2A-CD90.2 | Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the LTR promoter |
| 163339 | pMY-Ly6G-Rluc_miR | Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker |
| 163340 | pMY-Ly6G-P2A-PuroR-HA-Rluc_miR | Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection marker |
| 163341 | pMY-CD90.2-P2A-BlastDYK-Rluc_miR | Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and a DYK-tagged Blasticidin selection marker |
| 163342 | pMY-mCherry-P2A-PuroR-HA | Retroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection marker |
| 163343 | pMY-eGFP-P2A-PuroR-HA | Retroviral vector to express cytosolic GFP and an HA-tagged puromycin selection marker |
| 163344 | pMY-mTurquoise2-P2A-PuroR-HA | Retroviral vector to express cytosolic mTurquoise2 and an HA-tagged puromycin selection marker |
| 163345 | pMY-mVenus-P2A-PuroR-HA | Retroviral vector to express cytosolic mVenus and an HA-tagged puromycin selection marker |
| 163346 | pMY-Lyz2-GSG-mCherry | Retroviral vector to express C-terminally mCherry-tagged murine Lyz2 |
| 163347 | pMY-Lyz2-GSG-mTurquoise | Retroviral vector to express C-terminally mTurquoise2-tagged murine Lyz2 |
| 163348 | pMY-Lyz2-GSG-mVenus | Retroviral vector to express C-terminally mVenus-tagged murine Lyz2 |
| 163349 | pMY-CD90.2-P2A-BlastDYK | Retroviral vector to express the surface marker CD90.2 and a DYK-tagged blasticidin resistance marker |
| 163350 | pMY-MCS-GSG-eGFP | Retroviral backbone for in-frame cloning of a C-terminally GFP labeled gene of interest. |
| 163351 | pMY-empty-MCS | The empty pMY backbone with multiple cloning site |
| 163352 | pMY-MCS-P2A-PuroR-HA | Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection marker |
| 163353 | pMY-MCS-P2A-Ly6G | Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Ly6G surface marker |
| 163354 | pMY-MCS-P2A-CD90.1 | Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.1 surface marker |
| 163355 | pMY-MCS-P2A-CD90.2 | Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.2 surface marker |
| 163356 | pMY-MCS-P2A-eGFP | Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP marker |
| 163357 | pMY-MCS-P2A-BlastR-DYK | Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Blasticidin selection marker |
| 163358 | pLenti-EF1a-Ly6G-miR30 | Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker. |
| 163359 | pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30 | Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker. |
| 163360 | pLenti-EF1a-CD90.2-Rluc_miR | Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a CD90.2 surface marker. |
| 163361 | pMY-IRES-eGFP | Retroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP marker |
| 163362 | pLenti-EFS-MCS-WPRE | The empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectors |