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Targeted gene disruption of ATP synthases 6-1 and 6-2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs.

Arimura SI, Ayabe H, Sugaya H, Okuno M, Tamura Y, Tsuruta Y, Watari Y, Yanase S, Yamauchi T, Itoh T, Toyoda A, Takanashi H, Tsutsumi N
Plant J. 2020 Oct 24. doi: 10.1111/tpj.15041. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
163145pDESTpK7WG-RPS5Ap-mitoPlant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter
163146pENTR E2 HSPter-RPS5Ap-mitoPlant mitoTALEN vector assembly, Entry clone 2 with attL1 L4; Multisite Gateway entry vector to terminate the E1 ORF and having RPS5A promoter with mitochondrial presequence for the E3 ORF

Antibodies from Article