Genome-wide CRISPR screens of oral squamous cell carcinoma reveal fitness genes in the Hippo pathway.
Chai AWY, Yee PS, Price S, Yee SM, Lee HM, Tiong VK, Goncalves E, Behan FM, Bateson J, Gilbert J, Tan AC, McDermott U, Garnett MJ, Cheong SC
Elife. 2020 Sep 29;9. pii: 57761. doi: 10.7554/eLife.57761.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163138 | pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W | Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag |
| 163147 | pKLV2-U6gRNA5(hCHAT)-PGKpuro2ABFP-W | Lentiviral gRNA plasmid targeting human CHAT gene, co-expression of BFP tag |
| 163169 | pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W | Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag |
| 163170 | pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-W | Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag |
| 163172 | pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W | Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag |
| 163174 | pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W | Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag |
| 163175 | pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W | Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag |
| 163176 | pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W | Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag |
| 163177 | pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W | Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag |
| 163178 | pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W | Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag |
| 163179 | pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W | Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag |