LyGo: A Platform for Rapid Screening of Lytic Polysaccharide Monooxygenase Production.
Hernandez-Rollan C, Falkenberg KB, Rennig M, Bertelsen AB, Ipsen JO, Brander S, Daley DO, Johansen KS, Norholm MHH
ACS Synth Biol. 2021 Apr 16;10(4):897-906. doi: 10.1021/acssynbio.1c00034. Epub 2021 Apr 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163127 | pLyGo-Ec-1 | pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI) |
| 163128 | pLyGo-Ec-2 | pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI) |
| 163129 | pLyGo-Ec-3 | pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI) |
| 163130 | pLyGo-Ec-4 | pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI) |
| 163131 | pLyGo-Ec-5 | pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI) |
| 163132 | pLyGo-Ec-6 | pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI) |
| 163135 | pLyGo-Ec-7 | pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI) |
| 163136 | pLyGo-Ec-8 | pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI) |
| 163137 | pLyGo-Bs-1 | pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI) |
| 163140 | pLyGo-Bs-2 | pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI) |
| 163141 | pLyGo-Bs-3 | pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI) |
| 163142 | pLyGo-Bs-4 | pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI) |
| 163143 | pLyGo-Kp-1 | pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI) |
| 163144 | pLyGo-Kp-2 | pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI) |