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Imaging living obligate anaerobic bacteria with bilin-binding fluorescent proteins.

Chia HE, Zuo T, Koropatkin NM, Marsh ENG, Biteen JS
Curr Res Microb Sci. 2020 Sep;1:1-6. doi: 10.1016/j.crmicr.2020.04.001. Epub 2020 May 11. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
163124pBAD_IPF2.0A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.
163125pMAL-c5x_UnaGA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.

Antibodies from Article