Optimization of S. aureus dCas9 and CRISPRi Elements for a Single Adeno-Associated Virus that Targets an Endogenous Gene.
Backstrom JR, Sheng J, Wang MC, Bernardo-Colon A, Rex TS
Mol Ther Methods Clin Dev. 2020 Sep 6;19:139-148. doi: 10.1016/j.omtm.2020.09.001. eCollection 2020 Dec 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163020 | (326) pAAV SV40-ZsGreen U6-gRNA | Fluorescent reporter for Sa gRNA (Bsa1 sites) |
| 163021 | (401) pAAV CMV-ZsGreen U6-gRNA | Fluorescent reporter for Sa gRNA (Bsa1 sites) |
| 163022 | (364) SB KRAB-SadCas9 | Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA) |
| 163023 | (517) SB KRAB-SadCas9 U6-gStop | Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA |
| 163024 | (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA | Expression of CRISPRi with U6-gRNA (Bsa1 sites) |
| 163025 | (564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9 | Expression of CRISPRi with gRNA against mouse Pcsk9 |
| 163030 | (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP | Expression of CRISPRi with gSTOP gRNA |