A quantitative study of the Golgi retention of glycosyltransferases.
Sun X, Mahajan D, Chen B, Song Z, Lu L
J Cell Sci. 2021 Oct 15;134(20). pii: 272560. doi: 10.1242/jcs.258564. Epub 2021 Oct 21.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163645 | Man1B1-Myc | Expresses myc tagged Man1B1 in mammalian cells |
| 163646 | Man1B1-GFP | Expresses GFP tagged Man1B1 in mammalian cells |
| 163647 | MGAT1-GFP | Expresses GFP tagged MGAT1 in mammalian cells |
| 163648 | Man2a1-myc | Expresses myc tagged Man2a1 in mammalian cells |
| 163649 | Man2a1-GFP | Expresses GFP tagged Man2a1 in mammalian cells |
| 163654 | FSS-GFP | Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR and TMD and luminal domain from ST6GAL1 in mammalian cells |
| 163655 | SFS-GFP | Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TFR and luminal domain from ST6GAL1 in mammalian cells |
| 163656 | SSF-GFP | Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TFR in mammalian cells |
| 163657 | SFF-GFP | Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TFR in mammalian cells |
| 163658 | FSF-GFP | Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR, TMD from ST6GAL1, and luminal domain from TFR in mammalian cells |
| 163659 | FFS-GFP | Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TFR, and luminal domain from ST6GAL1 in mammalian cells |
| 163660 | TNFa-GFP | Expresses GFP tagged TNFa in mammalian cells |
| 163661 | NSS-GFP | Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cells |
| 163662 | SNS-GFP | Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TNF and luminal domain from ST6GAL1 in mammalian cells |
| 163663 | SSN-GFP | Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TNF in mammalian cells |
| 163664 | SNN-GFP | Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TNF in mammalian cells |
| 163665 | NSN-GFP | Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF, TMD from ST6GAL1 and luminal domain from TNF in mammalian cells |
| 163666 | NNS-GFP | Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TNF, and luminal domain from ST6GAL1 in mammalian cells |
| 163667 | ST*-GFP | Expresses partial length ST in mammalian cells |
| 163668 | SBP-ST*-GFP | Expresses SBP tagged partial length ST in mammalian cells |
| 163669 | FKBP-ST*-GFP | Expresses FKBP tagged partial length ST in mammalian cells |
| 163670 | F9SS-GFP | Expresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, and TMD and luminal domain from ST6GAL1 in mammalian cells |
| 163671 | F9SF-GFP | Expresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from ST6GAL1 and luminal domain from TFR in mammalian cells |
| 163672 | F9FS-GFP | Expresses a TFR and ST6GAL1 swapped chimera with a truncated cytosolic tail from TFR, TMD from TFR and luminal domain from ST6GAL1 in mammalian cells |
| 163673 | TMD18 | Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells |
| 163674 | TMD19 | Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells |
| 163675 | TMD20 | Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells |
| 163676 | TMD21 | Expresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cells |
| 163677 | TMD22 | Expresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cells |
| 163678 | TMD24 | Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells |
| 163679 | F9F17F-GFP | Expresses a TFR mutant with truncated cytosolic tail and TMD in mammalian cells |
| 163680 | ss-ED-mCherry-GPI | Exprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cells |
| 163858 | VPS35 shRNA1 | shRNA targeting the coding sequences of VPS35 |
| 163859 | VPS35 shRNA2 | shRNA targeting the coding sequences of VPS35 |
| 163860 | GOLPH3 shRNA | shRNA targeting the coding sequences of GOLPH3 |
| 163861 | COG4 shRNA | shRNA targeting the coding sequences of COG4 |