High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.

Sinegubova MV, Orlova NA, Kovnir SV, Dayanova LK, Vorobiev II
PLoS One. 2021 Feb 2;16(2):e0242890. doi: 10.1371/journal.pone.0242890. eCollection 2021. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
162782	p1.1-Tr2-eGFP	Fluorescent reporter for protein expression studies
162783	pTM	Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded
162785	pTM-RBDv2	SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells