Retrograde transport of CDMPR depends on several machineries as analyzed by sulfatable nanobodies.
Buser DP, Bader G, Spiess M
Life Sci Alliance. 2022 Mar 21;5(7). pii: 5/7/e202101269. doi: 10.26508/lsa.202101269. Print 2022 Mar.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162777 | pET24a-LaM4-3xTS | Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope |
| 162778 | pET24a-LaM4-1xTS | Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope |
| 162779 | pET24a-LaM4-2xTS | Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope |
| 182641 | pET24a-LaM4-EGFP | Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope |
| 182642 | pQCXIP-EGFP-CDMPR | Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide) |
| 182643 | pQCXIP-mCherry-CDMPR | Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide) |
| 182644 | pQCXIP-cathepsinD-YMH | Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope |
| 182645 | pQCXIP-SHMY-PAUF | Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence |
| 182648 | pQCXIP-SHMY-A1Pi | Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and A1Pi sequence |