BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors.
Talas A, Simon DA, Kulcsar PI, Varga E, Krausz SL, Welker E
Nat Commun. 2021 Nov 3;12(1):6353. doi: 10.1038/s41467-021-26461-y.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162986 | pAT9624-BEAR-cloning | BEAR target cloning plasmid with split EGFP |
| 162987 | pAT9658-sgRNA-mCherry | sgRNA cloning plasmid |
| 162988 | pAT9679-sgRNA-BFP | sgRNA cloning plasmid |
| 162989 | pAT9651-BEAR-GFP | BEAR target plasmid with split EGFP and disrupted 5' splice site |
| 162990 | pAT9750-BEAR-mCherry | BEAR target plasmid with split mCherry and disrupted 5' splice site |
| 162991 | pAT9752-BEAR-mScarlet | BEAR target plasmid with split mScarlet and disrupted 5' splice site |
| 162992 | pAT9650-BEAR-GFP-preedited | BEAR control plasmid with split EGFP and intact 5' splice site |
| 162993 | pAT9751-BEAR-mCherry-preedited | BEAR control plasmid with split mCherry and intact 5' splice site |
| 162994 | pAT9753-BEAR-mScarlet-preedited | BEAR control plasmid with split mScarlet and intact 5' splice site |
| 162995 | pAT15415-BEAR-GFP-target-mCherry | plasmid expressing an sgRNA targeting the BEAR-GFP plasmid along with an mCherry marker |
| 162996 | pAT15416-BEAR-mScarlet-target-BFP | plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker |
| 162997 | pAT9676-ABE | plasmid expressing ABE |
| 162998 | pAT9749-dABE | plasmid expressing ABE with dCas9 |
| 162999 | pAT9991-eABE | plasmid expressing eABE |
| 163000 | pAT9992-HF-ABE | plasmid expressing HF-ABE |
| 163001 | pAT9993-Hypa-ABE | plasmid expressing Hypa-ABE |
| 163002 | pAT9994-HypaR661A-ABE | plasmid expressing HypaR661A-ABE |
| 163003 | pAT9995-evoABE | plasmid expressing evoABE |
| 163004 | pAT9996-HeF-ABE | plasmid expressing HeF-ABE |
| 163005 | pAT9784-xABE | plasmid expressing xABE |
| 163006 | pAT15002-ABEmax | plasmid expressing ABEmax |
| 163007 | pAT9675-CBE | plasmid expressing CBE |
| 163008 | pAT9748-dCBE | plasmid expressing CBE with dCas9 |
| 163009 | pAT15064-eCBE | plasmid expressing eCBE |
| 163010 | pAT15065-HF-CBE | plasmid expressing HF-CBE |
| 163011 | pAT15066-Hypa-CBE | plasmid expressing Hypa-CBE |
| 163012 | pAT15067-HypaR661A-CBE | plasmid expressing HypaR661A-CBE |
| 163013 | pAT15068-evoCBE | plasmid expressing evoCBE |
| 163014 | pAT15069-HeF-CBE | plasmid expressing HeF-CBE |
| 163015 | pAT15000-BE4max | plasmid expressing BE4max |
| 163016 | pAT15001-AncBE4max | plasmid expressing AncBE4max |
| 174082 | pAT15516_BEAR-GFP-2in1 | BEAR-GFP plasmid with a gRNA that targets its own plasmid |
| 174120 | pAT15482_ABE8e | Expresses ABE8e with an EF1a promoter |
| 174121 | pAT15483_e-ABE8e | Expresses ABE8e fused with eCas9 |
| 174122 | pAT15484_HF-ABE8e | Expresses ABE8e fused with SpCas9-HF1 |
| 174123 | pAT15485_Hypa-ABE8e | Expresses ABE8e fused with Hypa-SpCas9 |
| 174124 | pAT15486_HypaR661A-ABE8e | plasmid expressing HypaR661A-ABE8e |
| 174125 | pAT15487_evo-ABE8e | plasmid expressing evo-ABE8e |
| 174126 | pAT15488_HeF-ABE8e | plasmid expressing HeF-ABE8e |
| 174127 | pAT15489_dABE8e | plasmid expressing ABE8e with dCas9 |