In vivo Validation of Bimolecular Fluorescence Complementation (BiFC) to Investigate Aggregate Formation in Amyotrophic Lateral Sclerosis (ALS).
Don EK, Maschirow A, Radford RAW, Scherer NM, Vidal-Itriago A, Hogan A, Maurel C, Formella I, Stoddart JJ, Hall TE, Lee A, Shi B, Cole NJ, Laird AS, Badrock AP, Chung RS, Morsch M
Mol Neurobiol. 2021 May;58(5):2061-2074. doi: 10.1007/s12035-020-02238-0. Epub 2021 Jan 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162608 | pCS2plus-TDP-43 | Allows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assays |
| 162609 | pCS2plus-TDP-43 M337V | Allows for transcription of mutant TDP-43-M337V mRNA for injection into zebrafish embryos for BiFC assays |
| 162610 | pCS2plus-mVenus1-155-GGGS | Allows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assays |
| 162611 | pCS2plus-mVenus1-155-GGGS-TDP-43 | Allows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays |
| 162612 | pCS2plus-mVenus1-155-GGGS-TDP43 M337V | Allows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays |
| 162613 | pCS2plus-mVenus1-155-I152L-GGGS | Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays |
| 162614 | pCS2plus-mVenus1-155-I152L-GGGS-TDP-43 | Allows for transcription of improved mVenus I152L fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays |
| 162615 | pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337V | Allows for transcription of improved mVenus I152L fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays |
| 162616 | pCS2plus-mCerulean 156-239-GGGS | Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays |
| 162617 | pCS2plus-mCerulean 156-239-GGGS-TDP-43 | Allows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays |
| 162618 | pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V | Allows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays |
| 162624 | pCS2plus-mKate2 | Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays |