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CRISPR-Cas9 Gene Editing in Yeast: A Molecular Biology and Bioinformatics Laboratory Module for Undergraduate and High School Students.

Sankaran SM, Smith JD, Roy KR
J Microbiol Biol Educ. 2021 May 31;22(2):e00106-21. doi: 10.1128/jmbe.00106-21. eCollection 2021 Fall. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
162602pG1Edit Ade2 gene in yeast
162603pG2Edit Ade2 gene in yeast
162604pG3Edit Ade2 gene in yeast
162605pG4Edit Ade2 gene in yeast
162606pCas9Pre-express Cas9 in yeast
162607pEmpty_ControlEmpty control for Cas9 experiments and to clone new guide RNAs for paper

Antibodies from Article