Kinetic and Thermodynamic Insights into Agonist Interactions with the Parathyroid Hormone Receptor-1 from a New NanoBRET Assay.

Yu Z, Cary BP, Kim TW, Nguyen KD, Gardella TJ, Gellman SH
ACS Chem Biol. 2022 Nov 18;17(11):3148-3158. doi: 10.1021/acschembio.2c00595. Epub 2022 Oct 25. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
162577	nLuc-PTHR1	Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells
190011	nLuc-ΔECD-PTHR1	Expresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domain