Characterizing the portability of phage-encoded homologous recombination proteins.
Filsinger GT, Wannier TM, Pedersen FB, Lutz ID, Zhang J, Stork DA, Debnath A, Gozzi K, Kuchwara H, Volf V, Wang S, Rios X, Gregg CJ, Lajoie MJ, Shipman SL, Aach J, Laub MT, Church GM
Nat Chem Biol. 2021 Jan 18. pii: 10.1038/s41589-020-00710-5. doi: 10.1038/s41589-020-00710-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162572 | pARC8-LambdaRedBeta-EcSSB | Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. |
| 162576 | pARC8-LambdaRedBeta-EcSSB-EcMutLE32K | Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein. |