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Rational design and implementation of a chemically inducible heterotrimerization system.

Wu HD, Kikuchi M, Dagliyan O, Aragaki AK, Nakamura H, Dokholyan NV, Umehara T, Inoue T
Nat Methods. 2020 Sep;17(9):928-936. doi: 10.1038/s41592-020-0913-x. Epub 2020 Aug 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
162425Lyn-YFP-FRB1Cfor use in CIT (chemically induced trimerization) system
162426Lyn-YFP-FRB2Cfor use in CIT (chemically induced trimerization) system
162427Lyn-YFP-FRB3Cfor use in CIT (chemically induced trimerization) system
162428FRB1N-CFP-CAAXfor use in CIT (chemically induced trimerization) system
162429FRB2N-CFP-CAAXfor use in CIT (chemically induced trimerization) system
162430FRB3N-CFP-CAAXfor use in CIT (chemically induced trimerization) system
162431Lyn-YFP-FRB1Nfor use in CIT (chemically induced trimerization) system
162432mCh-FRB1C-Cb5for use in CIT (chemically induced trimerization) system
162433CFP-FKBPfor use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system
162434Lyn-CFP-FRB1C_no-linkerfor use in CIT (chemically induced trimerization) system
162435YFP-FRB1N-Cb5for use in CIT (chemically induced trimerization) system
162436Tom20-mCh-FKBPfor use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system
162437Lyn-YFP-FKBP1Nfor use in CIT (chemically induced trimerization) system
162438FKBP1C-CFP-Cb5for use in CIT (chemically induced trimerization) system
162439Lyn-YFP-Clover-FRB1Nfor use in CIT (chemically induced trimerization) system
162440mRuby-PH-PLCdeltafor use in CIT (chemically induced trimerization) system
162441iRFP-FRB1C-Cb5for use in CIT (chemically induced trimerization) system

Antibodies from Article