An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification.
Cai H, Yao H, Li T, Hutter CAJ, Li Y, Tang Y, Seeger MA, Li D
Commun Biol. 2020 Dec 10;3(1):753. doi: 10.1038/s42003-020-01478-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159418 | pETSG | Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli |
| 159419 | pYTSG | Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae |
| 159420 | pBTSG | Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells |
| 159421 | pSB_init-Sb44 | Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44 |
| 159422 | pSB_init-Sb66 | Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66 |
| 159423 | pSB_init_Sb68 | Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68 |
| 159424 | pSB_init_Sb92 | Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92 |
| 162389 | pFTSG | Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell |