Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens.
Black JB, McCutcheon SR, Dube S, Barrera A, Klann TS, Rice GA, Adkar SS, Soderling SH, Reddy TE, Gersbach CA
Cell Rep. 2020 Dec 1;33(9):108460. doi: 10.1016/j.celrep.2020.108460.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162333 | pLV_hUbC-dSpCas9-2xVP64-T2A-BSD | Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance |
| 162334 | pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR | Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance |
| 162335 | pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR | Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker |
| 162336 | pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP | Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker |
| 162337 | pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP | Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker |
| 162338 | pTet-O-NEUROD1-T2A-PuroR | Lentiviral expression of NEUROD1 under the control of the TetON promoter |
| 162339 | pTet-O-NEUROG1-T2A-PuroR | Lentiviral expression of NEUROG1 under the control of the TetON promoter |
| 162341 | pTet-O-NEUROG3-T2A-PuroR | Lentiviral expression of NEUROG3 under the control of the TetON promoter |
| 162342 | pTet-O-ATOH1-T2A-PuroR | Lentiviral expression of ATOH1 under the control of the TetON promoter |
| 162343 | pTet-O-ATOH7-T2A-PuroR | Lentiviral expression of ATOH7 under the control of the TetON promoter |
| 162345 | pTet-O-ASCL1-T2A-PuroR | Lentiviral expression of ASCL1 under the control of the TetON promoter |
| 162346 | pTet-O-KLF7-T2A-PuroR | Lentiviral expression of KLF7 under the control of the TetON promoter |
| 162347 | pTet-O-OVOL1-T2A-PuroR | Lentiviral expression of OVOL1 under the control of the TetON promoter |
| 162348 | pTet-O-E2F7-T2A-PuroR | Lentiviral expression of E2F7 under the control of the TetON promoter |
| 162349 | pTet-O-RUNX3-T2A-PuroR | Lentiviral expression of RUNX3 under the control of the TetON promoter |
| 162350 | pTet-O-LHX8-T2A-PuroR | Lentiviral expression of LHX8 under the control of the TetON promoter |