Biofoundry-assisted expression and characterization of plant proteins.
Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron NJ
Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 10.1093/synbio/ysab029. eCollection 2021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162281 | pEPQD0KN0024 | Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis |
| 162282 | pEPQD0KN0244 | Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis |
| 162283 | pEPQD0KN0025 | Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis |
| 162284 | pEPQD0KN0245 | Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis |
| 162285 | pEPQD0CB0026 | Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System |
| 162286 | pEPQD0CB0246 | Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System |
| 162287 | pEPQD0KN0282 | Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System |
| 162288 | pEPQD0KN0283 | Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System |
| 162289 | pEPQD0KN0284 | Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System |
| 162290 | pEPQD0KN0285 | Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System |
| 162291 | pEPYC0CM0258 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162292 | pEPQD0CM0541 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162293 | pEPQD0CM0542 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162294 | pEPQD0CM0543 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162295 | pEPQD0CM0544 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162296 | pEPQD0CM0545 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162297 | pEPQD0CM0546 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162298 | pEPQD0CM0547 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162299 | pEPQD0CM0548 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162300 | pEPQD0CM0549 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162301 | pEPQD0CM0550 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162302 | pEPQD0CM0281 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162303 | pEPQD0CM0551 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162304 | pEPMY0SP0002 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 162305 | pEPQD0CM0296 | Level 0 CDS part for MoClo assembly, AATG-TTCG |
| 162306 | pEPQD0CM0539 | Level 0 CDS part for MoClo assembly, AATG-TTCG |
| 162307 | pEPYC0CM0134 | Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT |
| 162308 | pEPYC0CM0257 | Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT |
| 162309 | pEPQD0CM0027 | Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT |
| 162310 | pEPQD0CM0028 | Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT |
| 162311 | pEPQD0CM0029 | Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT |
| 162312 | pEPQD0CM0030 | Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT |
| 162313 | pEPQDKN0248 | Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT |
| 162314 | pEPQDKN0332 | Reporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiT |
| 162315 | pEPQDKN0329 | TEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT |
| 162316 | pEPQDKN0729 | TEV protease plasmid for T7-driven E. coli cell-free protein synthesis |
| 162317 | pEPMY1CB0001 | Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter. |