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Cryptic prokaryotic promoters explain instability of recombinant neuronal sodium channels in bacteria.

DeKeyser JM, Thompson CH, George AL Jr
J Biol Chem. 2021 Jan-Jun;296:100298. doi: 10.1016/j.jbc.2021.100298. Epub 2021 Jan 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
162278pIR-CMV-SCN1A-Variant-1-IRES-mScarletEukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
162279pIR-CMV-SCN2A-Variant-1-IRES-mScarletEukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
162280pcDNA4TO-SCN8A-Variant-1-IRES-mScarletEukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Antibodies from Article