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High resolution biosensor to test the capping level and integrity of mRNAs.

Moya-Ramirez I, Bouton C, Kontoravdi C, Polizzi K
Nucleic Acids Res. 2020 Dec 16;48(22):e129. doi: 10.1093/nar/gkaa955. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
162067pET28a-B4EFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E

Antibodies from Article