An ARF GTPase module promoting invasion and metastasis through regulating phosphoinositide metabolism.
Nacke M, Sandilands E, Nikolatou K, Roman-Fernandez A, Mason S, Patel R, Lilla S, Yelland T, Galbraith LCA, Freckmann EC, McGarry L, Morton JP, Shanks E, Leung HY, Markert E, Ismail S, Zanivan S, Blyth K, Bryant DM
Nat Commun. 2021 Mar 12;12(1):1623. doi: 10.1038/s41467-021-21847-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162016 | pLEX304-GFP-v1 IQSEC1 WT | Expression of IQSEC1 splice variant 1 |
| 162017 | pLEX304-GFP-v2 IQSEC1 WT | Expression of IQSEC1 splice variant 2 |
| 162018 | pLEX304-GFP-v3 IQSEC1 WT | Expression of IQSEC1 splice variant 3 |
| 162019 | pLEX304-GFP-v4 IQSEC1 WT | Expression of IQSEC1 splice variant 4 |
| 162020 | pLEX304-GFP-IQSEC1 v2-E620K GEF dead mutant | Expression of GEF dead IQSEC1 mutant |
| 162022 | pLEX303-GFP-IQSEC1 v2N/v4C-WT | Expression of IQSEC1 chimera |
| 162023 | pLEX303-GFP-IQSEC1 v2N/v1C-WT | Expression of IQSEC1 chimera |
| 162024 | pLEX303-GFP-IQSEC1 v4N/v1C-WT | Expression of IQSEC1 chimera |
| 162025 | pLEX304-ARF5-mNeonGreen | Expression of tagged ARF WT |
| 162026 | pLEX304-ARF5-T161A-mNeonGreen | Fast cycling ARF mutant |
| 162027 | pLEX303-ARF6-mNeonGreen | Expression of tagged ARF WT |
| 162028 | pLEX303-ARF6-T157N-mNeonGreen | Fast cycling ARF mutant |
| 162029 | pLEX301-ARF6-TagRFP | Expression of tagged ARF WT |
| 162030 | pLEX301-ARF6-T157N-TagRFP | Fast cycling ARF mutant |
| 162031 | pLEX301-RFP-GGA3 NGAT | Expresses NGAT sequence of GGA3 |
| 162032 | pLEX303-GFP | Tagged vector control |
| 162033 | pLEX304-GFP | Tagged vector control |
| 162034 | pLEX304-mNeonGreen | Tagged vector control |
| 162035 | pLEX301-TagRFP | Tagged vector control |