In vivo screens using a selective CRISPR antigen removal lentiviral vector system reveal immune dependencies in renal cell carcinoma.

Dubrot J, Lane-Reticker SK, Kessler EA, Ayer A, Mishra G, Wolfe CH, Zimmer MD, Du PP, Mahapatra A, Ockerman KM, Davis TGR, Kohnle IC, Pope HW, Allen PM, Olander KE, Iracheta-Vellve A, Doench JG, Haining WN, Yates KB, Manguso RT
Immunity. 2021 Jan 20. pii: S1074-7613(21)00001-7. doi: 10.1016/j.immuni.2021.01.001. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
162078	pSCAR_sgRNA_hygro-tagBFP-lox2272	3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance
162077	pSCAR_sgRNA_puro-mKate-lox5171	3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance
162076	pSCAR_sgRNA_puro-mKate-lox2272	3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance
162075	pSCAR_Cas9-hygro_GFP	3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance
162074	pSCAR_Cas9-blast_GFP	3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
162073	pLX_EFS-Cre_ppt-del	Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)
162072	pSCAR_sgRNA_blast-tagBFP-lox5171	3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance
162071	pSCAR_sgRNA_blast-tagBFP-lox2272	3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance
162070	pSCAR_sgRNA_hygro-tagBFP-lox5171	3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance