A systematic approach to inserting split inteins for Boolean logic gate engineering and basal activity reduction.
Ho TYH, Shao A, Lu Z, Savilahti H, Menolascina F, Wang L, Dalchau N, Wang B
Nat Commun. 2021 Apr 13;12(1):2200. doi: 10.1038/s41467-021-22404-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 161937 | IBMc090 | Carries engineered transposon for IBM |
| 161938 | IBMc050 | Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter |
| 161939 | IBMc233 | Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter |
| 161940 | IBMc460 | Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter |
| 161941 | IBMc318 | Carries Golden Gate substitution insert for ER-LBD domain-insertion mapping |
| 161942 | IBMc180 | Carries Golden Gate substitution insert for acVHH-assisted bisection mapping |
| 161943 | IBMc071 | Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid |
| 161944 | IBMc226 | Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid |
| 161945 | IBMc097 | Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry |
| 161946 | IBMc228 | Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetR |
| 161947 | IBMc367 | Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20 |
| 161948 | IBMc227 | Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetR |
| 161949 | IBMc326 | Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20 |
| 161950 | IBMc120 | Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid |
| 161951 | IBMc259 | Contains elements for building reporter plasmid for IBM.TetR reporter plasmid |
| 161952 | IBMc330 | Contains elements for building reporter plasmid for IBM. SrpR reporter plasmid |
| 161953 | IBMc393 | Demonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmid |
| 161954 | IBMc396 | Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH |
| 161955 | IBMc397 | Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH |