Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate phosphate.
Miller SS, Dornbusch MR, Farmer AD, Huertas R, Gutierrez-Gonzalez JJ, Young ND, Samac DA, Curtin SJ
G3 (Bethesda). 2022 May 30;12(6). pii: 6574359. doi: 10.1093/g3journal/jkac096.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 161760 | pMOD_B-Pho2-1/2-2-Csy4 | MODULE B Csy4 vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2103 - #91061) |
| 161761 | pMOD_B-Pho2-1/2-2-tRNA | MODULE B tRNA vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2303 - #91068) |
| 161762 | pTRANS-Pho2-1/2-2-tRNA | pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113) |
| 161763 | pTRANS-Pho2-1/2-2-Csy4 | pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113) |
| 161764 | pMOD_C2911 | MODULE C plasmid compatible with the Voytas Plant Genome Engineering Tool kit with an Agrobacterium promoter rolD expressing the TREX2 exonuclease. |