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Dark, Ultra-Dark and Ultra-Bright Nanodiscs for membrane protein investigations.

McLean MA, Denisov IG, Grinkova YV, Sligar SG
Anal Biochem. 2020 Oct 15;607:113860. doi: 10.1016/j.ab.2020.113860. Epub 2020 Aug 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
161607pD-MSP1D1Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation
161608pUD-MSP1D1Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation
161609pUB-MSP1D1Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

Antibodies from Article