Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment.
Natwick DE, Collins SR
ACS Synth Biol. 2021 May 21;10(5):1009-1023. doi: 10.1021/acssynbio.0c00511. Epub 2021 Apr 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160999 | pLV_mTurquoise2-iLID-CAAX | Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS |
| 161000 | pLV_Lyn11-mTurquoise2-iLID | Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN |
| 161001 | pLV_Stargazin-mTurquoise2-iLID | Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion |
| 161002 | pLV_ADRB2-mTurquoise2-iLID | Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion |