Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli.
Lin MT, Stone WD, Chaudhari V, Hanson MR
Nat Plants. 2020 Oct;6(10):1289-1299. doi: 10.1038/s41477-020-00761-5. Epub 2020 Sep 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160873 | pET-NtLAtC60αβC20 | Expression of N. tabacum Rubisco large subunit and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli |
| 160874 | pET-NtLNtC60αC20C60β | Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli |
| 160875 | pET-NtLNtC60αESC60β | Expression of N. tabacum Rubisco large subunit (rbcL), Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli |
| 160876 | pET-AtC60αβC20NtL | Expression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli |
| 160877 | pET-NtC60αC20NtLC60β | Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli |
| 160878 | pET-NtC60αESNtLC60β | Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli. |
| 160879 | pCDF-NtXR1AtR2B2NtS1 | Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana Rubisco chaperone proteins raf2, and bsd2 in E. coli. |
| 160880 | pCDF-NtXNtS1R1AtR2B2 | Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli. |
| 160881 | pCDF-NtXNtS1R1AtR2NtB2 | Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli. |
| 160882 | pCDF-NtXNtS1R1R2B2 | Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli. |
| 160883 | pCDF-NtXNtS2R1AtR2NtB2 | Expression of N. tabacum Rubisco small subunit (rbcS-S2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli. |
| 160884 | pCDF-NtXNtS5R1AtR2NtB2 | Expression of N. tabacum Rubisco small subunit (rbcS-S5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli. |
| 160885 | pCDF-NtXNtT1R1AtR2NtB2 | Expression of N. tabacum Rubisco small subunit (rbcS-T1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli. |
| 160886 | pCDF-NtXNtT2R1AtR2NtB2 | Expression of N. tabacum Rubisco small subunit (rbcS-T2), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli. |
| 160887 | pCDF-NtXNtT4aR1AtR2NtB2 | Expression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli. |
| 160888 | pCDF-NtXNtT5R1AtR2NtB2 | Expression of N. tabacum Rubisco small subunit (rbcS-T5), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli. |
| 160889 | pCDF-NtXNtTcR1AtR2NtB2 | Expression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli. |
| 160890 | pCDF-NtXNtS1HR1AtR2NtB2 | Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli. |