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Synthetic protein-binding DNA sponge as a tool to tune gene expression and mitigate protein toxicity.

Wan X, Pinto F, Yu L, Wang B
Nat Commun. 2020 Nov 24;11(1):5961. doi: 10.1038/s41467-020-19552-9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
160818pXW117Ptet2-gfpaTc-responsive single-layered circuit
160819pXW101Plux2-gfpAHL-responsive single-layered circuit
160820pXW101Plux2-33Ptet2-gfpTwo-layered AHL-responsive circuit containing a TetR-based inverter
160821pXW101Plux2-32Ptet2-gfpTwo-layered AHL-responsive circuit containing a TetR-based inverter
160822pXW109PmerT-Pecf11-gfpTwo-layered mercury responsive circuit carrying an ECF11-based amplifier
160823pXW101Plux2-Pecf11-gfpTwo-layered AHL-responsive circuit carrying an ECF11-based amplifier
160824pXWStetO1 repeat of tetO sponge
160825pXWS10tetO10 repeats of tetO sponge
160826pXWS20tetO20 repeats of tetO sponge
160827pXWS40tetO40 repeats of tetO sponge
160828pXWS80tetO80 repeat of tetO sponge
160829pXWS160tetO160 repeats of tetO sponge
160830pXWS320tetO320 repeats of tetO sponge
160831pXWSPtet21 repeat of Ptet2 sponge
160832pXWS5Ptet25 repeats of Ptet2 sponge
160833pXWS10Ptet210 repeats of Ptet2 sponge
160834pXWS20Ptet220 repeats of Ptet2 sponge
160835pXWS40Ptet240 repeats of Ptet2 sponge
160836pXWSLBS1 repeat of LBS sponge
160837pXWS10LBS10 repeats of LBS sponge
160838pXWS20LBS20 repeats of LBS sponge
160839pXWS40LBS40 repeats of LBS sponge
160840pXWS80LBS80 repeats of LBS sponge
160841pXWSPecf111 repeat of Pecf11 sponge
160842pXWS10Pecf1110 repeats of Pecf11 sponge
160843pXWS20Pecf1120 repeats of Pecf11 sponge
160844pXWS40Pecf1140 repeats of Pecf11 sponge
160845PXWS80LBS40tetOLBS-tetO hybrid sponge with 80 repeats of LBS and 40 repeats of tetO
160846PXWS80LBS320tetOLBS-tetO hybrid sponge with 80 repeats of LBS and 320 repeats of tetO
160847PXWS20LBS20Pecf11LBS-tetO hybrid sponge with 20 repeats of LBS and 20 repeat of Pecf11
160848PXWS80LBS40Pecf11LBS-tetO hybrid sponge with 80 repeats of LBS and 40 repeat of Pecf11

Antibodies from Article