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Kathleen Postle Lab: Postle Lab Plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
160815pKP1469Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
167596pKP1963Express ExbB delta(195-204),ExbD
167597pKP1964Express ExbB delta(205-214),ExbD
167598pKP1965Express ExbB delta(215-224),ExbD
167599pKP1940Express ExbB delta(225-234),ExbD
167600pKP1912Express ExbB delta(235-244),ExbD
167601pKP2008Express ExbB (205-214 Ala),ExbD
167602pKP2009Express ExbB (215-224 Ala),ExbD
167603pKP2010Express ExbB (235-244 Ala),ExbD
167604pKP2019Express ExbB (195-204 Ala),ExbD
167605pKP2025Express ExbB (225-234 Ala),ExbD
167606pKP2085Express ColicinB-V5-TEV-T7-mature MBP
167635pKP2185Express FepA D12C, I14P . Please note that mutation numbers are not based on start codon of insert.
167637pKP2186Express FepA T13C, I14P . Please note that mutation numbers are not based on start codon of insert.
167640pKP2187Express FepA V15C, I14P . Please note that mutation numbers are not based on start codon of insert.
167642pKP2188Express FepA V16C, I14P . Please note that mutation numbers are not based on start codon of insert.
167644pKP2189Express FepA T17C, I14P . Please note that mutation numbers are not based on start codon of insert.
167649pKP1392Express ExbD T42C protein
167650pKP2040Express His6-ExbB S80A, S82A, S84A
167651pKP2032Express His6-ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
167653pKP968Express colicinB-His6, D17C, M261C
167654pKP996Express colicinB-His6, T18C, M261C
167655pKP995Express colicinB-His6, M19C, M261C
167656pKP997Express colicinB-His6, V20C, M261C
167658pKP864Express colicinB-His6
167660pKP2264Express colicinB-His6, A121C, Y254C

Antibodies from Article