Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics.
Linghu C, Johnson SL, Valdes PA, Shemesh OA, Park WM, Park D, Piatkevich KD, Wassie AT, Liu Y, An B, Barnes SA, Celiker OT, Yao CC, Yu CJ, Wang R, Adamala KP, Bear MF, Keating AE, Boyden ES
Cell. 2020 Nov 17. pii: S0092-8674(20)31399-4. doi: 10.1016/j.cell.2020.10.035.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160722 | pAAV-UBC-S1-GCaMP6f | Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold. |
| 160723 | pAAV-UBC-S2-cAMPr | Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold. |
| 160724 | pAAV-UBC-S3-ExRaiAKAR | Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold. |
| 160725 | pAAV-UBC-S4-ExRaiCKAR | Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold. |
| 160726 | pAAV-UBC-S2-RAB_EKARev | Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold. |
| 160727 | pAAV-UBC-S3-RAB_EKARev | Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold. |
| 160728 | pAAV-UBC-S1-jRCaMP1a | Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold. |
| 161613 | pAAV-hSyn-mRuby3-6xFLAG | Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags. |
| 161614 | pAAV-hSyn-miRFP-6xFLAG | Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags. |