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Growth-related changes in phosphorylation of yeast RNA polymerase II.

Patturajan M, Schulte RJ, Sefton BM, Berezney R, Vincent M, Bensaude O, Warren SL, Corden JL
J Biol Chem. 1998 Feb 20;273(8):4689-94. doi: 10.1074/jbc.273.8.4689. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
160688pGEX-2T-RNAPII-CTD wt #14-HA Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.
160689pGEX-2T-RNAPII-A2-14-HA Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

Antibodies from Article