Programmable Gene Knockdown in Diverse Bacteria Using Mobile-CRISPRi.
Banta AB, Ward RD, Tran JS, Bacon EE, Peters JM
Curr Protoc Microbiol. 2020 Dec;59(1):e130. doi: 10.1002/cpmc.130.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160666 | pJMP2754 | Mobile CRISPRi sfGFP 'test', empty sgRNA |
| 160667 | pJMP2774 | Mobile CRISPRi sfGFP 'test', gmc6 sgRNA |
| 160668 | pJMP2782 | Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs |
| 160669 | pJMP2820 | Mobile CRISPRi sfGFP 'test', empty sgRNA |
| 160670 | pJMP2822 | Mobile CRISPRi sfGFP 'test', empty sgRNA |
| 160671 | pJMP2824 | Mobile CRISPRi sfGFP 'test', empty sgRNA |
| 160672 | pJMP2832 | Mobile CRISPRi sfGFP 'test', gmc6 sgRNA |
| 160673 | pJMP2834 | Mobile CRISPRi sfGFP 'test', gmc6 sgRNA |
| 160674 | pJMP2836 | Mobile CRISPRi sfGFP 'test', gmc6 sgRNA |
| 160675 | pJMP2844 | Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs |
| 160676 | pJMP2846 | Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs |
| 160677 | pJMP2849 | Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs |