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Engineering and Rewiring of a Calcium-Dependent Signaling Pathway.

Mesko M, Lebar T, Dekleva P, Jerala R, Bencina M
ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 10.1021/acssynbio.0c00133. Epub 2020 Jul 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
160661hNFAT2-409:TALE:VP64:KRɸConstitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.
160662mNFAT4-462:TALE:VP64:KRɸConstitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.
16066310TALE_Pmin_fLucExpression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain

Antibodies from Article