Diego Orzaez Lab: Orzaez lab plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160553 | Tobacco Etch Virus NIa protease (GB0420) | pUPD_NIaPro(TEV) |
| 160555 | pUPD Pnos (GB0555) | Nos promoter for N-terminal fusions |
| 160556 | pUPD DsRed-CT (GB1183) | DsRed for C-terminal fusions |
| 160569 | pUPD2 hpIntron (GB1281) | Intron sequence from S. lycopersicum |
| 160584 | pUPD2 AlcA (GB1549) | Ethanol inducible operator |
| 160585 | pUPD2 AlcR (GB1550) | Ethanol dependent transcrition factor |
| 160587 | pUPD2 nickase D10A Cas9 (GB1690) | Nickase D10A Cas9 for Nt and Ct fusion. |
| 160588 | pUPD2 nickase D10A Cas9 (GB1691) | Nickase D10A Cas9 for Nt fusion. |
| 160589 | pUPD2 NtSPrbcS (GB2213) | B3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chain |
| 160590 | pUPD2 GFP-NT (GB2236) | GFP for N-terminal fusions |
| 160591 | pUPD2 GFP (GB2237) | GFP coding sequence. |
| 160592 | pUPD2 GFP-CT (GB2238) | GFP for C-terminal fusions. |
| 160596 | pUPD2 dCas9 (GB2664) | CDS of dCas9 with B3 nomenclature |
| 160597 | pUPD2 MS2 (GB2665) | CDS of MS2 protein with B3 nomenclature |
| 160598 | pUPD2 ER-ER (GB2666) | two repeats of ER (Estradiol receptor) |
| 160599 | pUPD2 CUP2 (GB2716) | Copper dependent transcription factor |
| 160600 | pUPD2 VP16 (GB2717) | Transcriptional activator |
| 160601 | pUPD2 CBS4 (GB2718) | Copper dependent CUP2 binding DNA sequence |
| 160602 | pUPD2 Etr4 (GB2719) | |
| 160603 | pUPD2 PhyB (GB2721) | chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch. |
| 160607 | pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039) | TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription. |
| 160612 | GB_PVX (GB1464) | PVX vector adapted to GB |
| 160618 | pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656) | Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII). |
| 160629 | pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479) | TU for the expression of LbCas12a |
| 160630 | pEGB3_alpha2 35:PhyB:VP16 (GB2745) | TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch |
| 160631 | pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759) | Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter. |
| 160632 | pEGB3_alpha1 Pnos:P19:Tnos (GB2762) | TU for expression of P19 driven by Nos promoter. |
| 160634 | pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980) | TU for wtGFP expression driven by 2x35s promoter. |
| 160637 | pDGB3 alpha1 P35S:BFP:T35S (GB3416) | TU for the constitutive expression of Blue Fluorescent Protein |
| 160639 | AtS/MAR10 insulator sequence (GB3459) | pEGB3a2 AtS/MAR10 |
| 160640 | pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657) | Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII). |
| 160647 | pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469) | module containing the human codon optimized Cas9 and the BASTA selection marker |