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Assessing mycoplasma contamination of cell cultures by qPCR using a set of universal primer pairs targeting a 1.5 kb fragment of 16S rRNA genes.

Jean A, Tardy F, Allatif O, Grosjean I, Blanquier B, Gerlier D
PLoS One. 2017 Feb 22;12(2):e0172358. doi: 10.1371/journal.pone.0172358. eCollection 2017. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
160549p_m16SUsed for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: Carbenicilline

Antibodies from Article