Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins.

Jin F, Shen C, Wang Y, Wang M, Sun M, Hattori M
Commun Biol. 2021 Mar 19;4(1):366. doi: 10.1038/s42003-021-01891-y. PubMed Article

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ID	Plasmid	Purpose
160499	hP2X3-cALFA	For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells
160498	BbZIP-cALFA	For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli
159991	pBMNb-cALFA	For the protein expression with ALFA tag at C-terminus in mammalian cells
159990	pFBNb-cALFA	For the protein expression with ALFA tag at C-terminus in insect cells
159989	pETNb-cALFA	For the protein expression with ALFA tag at C-terminus in E. coli
159988	pETNb-nALFA	For the protein expression with ALFA tag at N-terminus in E. coli
159987	mCherry-NbALFA	For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)
159986	mEGFP-NbALFA	For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)