Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes.
Zhou T, Teng IT, Olia AS, Cerutti G, Gorman J, Nazzari A, Shi W, Tsybovsky Y, Wang L, Wang S, Zhang B, Zhang Y, Katsamba PS, Petrova Y, Banach BB, Fahad AS, Liu L, Lopez Acevedo SN, Madan B, Oliveira de Souza M, Pan X, Wang P, Wolfe JR, Yin M, Ho DD, Phung E, DiPiazza A, Chang LA, Abiona OM, Corbett KS, DeKosky BJ, Graham BS, Mascola JR, Misasi J, Ruckwardt T, Sullivan NJ, Shapiro L, Kwong PD
Cell Rep. 2020 Oct 27;33(4):108322. doi: 10.1016/j.celrep.2020.108322. Epub 2020 Oct 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160474 | pVRC8400-SARS-CoV-2-S2P-AVI | Expresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 160475 | pVRC8400-SARS-CoV-2-NTD-AVI | Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 160476 | pVRC8400-SARS-CoV-2-RBD-AVI | Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 160477 | pVRC8400-SARS-CoV-2-RBD-SD1-AVI | Expresses SARS-CoV-2 RBD-SD1 domain with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 160478 | pVRC8400-SARS-CoV-2-RBD-L455RA475R-AVI | Expresses SARS-CoV-2 RBD-L455RA475R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 160479 | pVRC8400-SARS-CoV-2-RBD-L455RG496R-AVI | Expresses SARS-CoV-2 RBD-L455RG496R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag |
| 160480 | pVRC8400-SARS-CoV-2-RBD-L455RA475RG502R-AVI | Expresses SARS-CoV-2 RBD-L455RA475RG502R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag |