Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem Cells.
Xi H, Young CS, Pyle AD
STAR Protoc. 2020 Nov 5;1(3):100158. doi: 10.1016/j.xpro.2020.100158. eCollection 2020 Dec 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160453 | pHX_gRNA_hPAX7_6-9S | sgRNA targeting human PAX7 3' UTR with 17bp spacer |
| 160454 | pCY_gRNA_hPAX7-Pro_C3 | sgRNA targeting human PAX7 promoter |
| 160455 | pCY_gRNA_hPAX7-Pro_C4 | sgRNA targeting human PAX7 promoter |
| 160456 | pCY_gRNA_hPAX7-Pro_C5 | sgRNA targeting human PAX7 promoter |
| 160457 | pCY_gRNA_hPAX7-Pro_C6 | sgRNA targeting human PAX7 promoter |
| 160458 | pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo | Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR |