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Use of Optogenetic Amyloid-beta to Monitor Protein Aggregation in Drosophila melanogaster, Danio rerio and Caenorhabditis elegans.

Kaur P, Kibat C, Teo E, Gruber J, Mathuru A, Tolwinski ANS
Bio Protoc. 2020 Dec 5;10(23):e3856. doi: 10.21769/BioProtoc.3856. eCollection 2020 Dec 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
160435pDEST-Tol2-PA2-CMV-AB-mChExpresses human Amyloid Beta-mCherry in Zebrafish
160436pUCIDT-attL1-Human ABeta-attR5Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination
160437pUCIDT-attL1-Worm ABeta-attR5Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination
160438pDONR-attL5-CRY2-mCh-attL2Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

Antibodies from Article