Use of Optogenetic Amyloid-beta to Monitor Protein Aggregation in Drosophila melanogaster, Danio rerio and Caenorhabditis elegans.
Kaur P, Kibat C, Teo E, Gruber J, Mathuru A, Tolwinski ANS
Bio Protoc. 2020 Dec 5;10(23):e3856. doi: 10.21769/BioProtoc.3856. eCollection 2020 Dec 5.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
160435 | pDEST-Tol2-PA2-CMV-AB-mCh | Expresses human Amyloid Beta-mCherry in Zebrafish |
160436 | pUCIDT-attL1-Human ABeta-attR5 | Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination |
160437 | pUCIDT-attL1-Worm ABeta-attR5 | Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination |
160438 | pDONR-attL5-CRY2-mCh-attL2 | Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination |