A high-throughput screening method for evolving a demethylase enzyme with improved and new functionalities.
Wang Y, Katanski CD, Watkins C, Pan JN, Dai Q, Jiang Z, Pan T
Nucleic Acids Res. 2021 Mar 18;49(5):e30. doi: 10.1093/nar/gkaa1213.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160433 | AlkB-D135T | A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr |
| 160434 | AlkB-D135G | A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly |