John McCusker Lab: Yeast viruses
Unpublished
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
160379 | pAG25-1199 cDNA | N1199 narnavirus cDNA |
160380 | pGAL-flp(H305L)-NAT | overexpression of Flp recombinase step-arrest mutant in yeast |
160381 | pESC-NAT-Ago-Dcr | Overexpression of Ago-Dcr fusion protein in yeast |
160382 | pGAL-SKI1-NAT | Overxpression of SKI1/XRN1 in yeast |
160384 | pCAS-mak10 gRNA-MAK10Δ | For CRISPR-mediated deletin og MAK10 in yeast |
160385 | pCAS-mak10 gRNA | Expresses guide RNA for targeting MAK10 in yeast |
160386 | N1199 launching | Expression of N1199 narnavirus ORF under PGK1 promoter with a 3' HDV ribozyme sequence |
160388 | pGAL-PDE1 | Overxpression of PDE1 in yeast |
160389 | GFP-ATG8(416)/GFP-AUT7(416) | Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection |
160390 | PJD1223 | Encodes L-A cDNA for curing L-A virus and Parent for pJD1223-NAT |