Ian Duncan Lab: Modified donor plasmids to enhance Golden Gate assembly of Drosophila two stage Crispr/Cas9 cassette exchange
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160229 | pXZ13lacZalpha | places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates |
| 160230 | pXZ13lacZalphaKanR | Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance. |