Simple, efficient and open-source CRISPR/Cas9 strategy for multi-site genome editing in Populus tremula x alba.
Triozzi P, Schmidt HW, Dervinis C, Kirst M, Conde D
Tree Physiol. 2021 May 7. pii: 6270869. doi: 10.1093/treephys/tpab066.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160218 | pGEM-T Easy-AtpU6-26 | AtpU6-26 Golden Gate level 0 piece to express gRNAs |
| 160219 | pGEM-T Easy-AtpU6-29 | AtpU6-29 Golden Gate level 0 piece to express gRNAs |
| 160220 | pICH47802-p35S::tdTomato-ER::tNOS | tdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverse |
| 160221 | pICH47811-pAtAct2::Cas9-nls::tSlATPase | Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse |
| 160231 | pGEM-T Easy-Cas9-nls-CDS1 | Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1 |